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The primary commercial indigo species in Asia was true indigo (Indigofera tinctoria, also known as I. A common alternative used in the relatively colder subtropical locations such as Japan's Ryukyu Islands and Taiwan is Strobilanthes cusia.

Dyer's knotweed (Polygonum tinctorum) was the most important blue dye in East Asia until the arrival of the Indigofera species from the south, which yield more dye.

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Indigo plantations also thrived in the Virgin Islands.A large percentage of indigo dye produced today – several thousand tons each year – is synthetic. The primary use for indigo is as a dye for cotton yarn, which is mainly for the production of denim cloth for blue jeans.On average, a pair of blue jean trousers requires 3–12 g of indigo. Indigo carmine, or indigo, is an indigo derivative which is also used as a colorant.The association of India with indigo is reflected in the Greek word for the dye, indikón (ινδικόν, Indian).The Romans latinized the term to indicum, which passed into Italian dialect and eventually into English as the word indigo.

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"In my opinion, it is hard to believe that in the past centuries, in a historical interval spanning the medieval period, different subjects — such as priests, monks or nuns, as well [as] devotees and other subjects of Indian ancestry — have had the possibility to come in contact with the shroud in France and/or Turin," Barcaccia said.

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