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Concomitant with a decrease in serum zinc concentration, changes in buccal and blood gene transcripts related to zinc homeostasis occurred with zinc depletion.

Microarray analyses of whole blood RNA revealed zinc-responsive genes, particularly, those associated with cell cycle regulation and immunity.

Responses of potential signature genes of dietary zinc depletion were further assessed by quantitative real-time PCR.

The diagnostic properties of specific serum micro RNAs for dietary zinc deficiency were identified by acute responses to zinc depletion, which were reversible by subsequent zinc repletion.

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In addition, the results of gene network analysis reveal potential clinical outcomes attributable to suboptimal zinc intake including immune function defects and predisposition to cancer.

These demonstrate through a controlled depletion/repletion dietary protocol that the illusive zinc biomarker(s) can be identified and applied to assessment and intervention strategies.

GRAND RAPIDS, MI - Lakewood School District is supporting "sexual predator, star athlete" Chad Curtis by asking if his teen victims "asked for it" when he sexually assaulted them at school, attorneys for the girls charge.

The district said the issue of consent, irrelevant in Curtis' criminal case, could be important for a jury hearing the million civil case against Curtis and the school district.

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