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Wear suitable protective clothing, eye/face protection and gloves. Different concentrations of anaesthetic solutions can be prepared in different bags that can be exchanged if necessary. Small amounts of anaesthetic fluid can be delivered directly to the gills via a bulb syringe without disconnecting the fish from the system. Induction usually takes 5-10 minutes and is marked by a decrease in caudal fin strokes, swimming, respiratory rate and reaction to stimuli. There is also a loss of equilibrium and the righting reflex is poor.
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